Rocky Mountain Neurological Disorders Center Core
Bill Betz, Core DirectorThe microscopy core has two components:
- Light Microscopy. The light microscopy facility is housed in RC-1 North East of the elevators. It houses a variety of microscopes including a Zeiss 510 confocal microscope that will be equipped with a metadetector for simultaneous
detection of multiple emission wavelengths with funds provided by our NINDS grant. The facility also includes a deconvolution microscope, a total internal reflection (TIRF) microscope and a rotating disk confocal (see Light Microscopy Core Facility for full description).
Individuals who want to use the light microscopy facility should contact Christine Romberg. Usage of the facility will be recorded, and access will be prioritized on usage history if demand exceeds capacity.
- Electron Microscopy. The electron microscopy facility is located in the first floor of RC-1 South (East of the elevators). A new a state-of-the-art TECNAI G1 12 BioTwin microscope was recently purchased with a SIG grant (Kathryn Howell,
P.I.). This microscope is currently being installed, and dates and times for training sessions will be announced shortly. Our grant provides partial salary coverage for Dot Dill, the electron microscopy technician, who will assist individuals
in sample preparation and use of the microscope. Individuals wishing to access the EM facility should contact Dot Dill.
Mark Dell'Acqua, Core DirectorThis core has two components:
- Generation of Gene-Targeted/Transgenic Mouse Constructs (Mark Dell'Acqua, Core B Director; Brain Parr, Coordinator; Wallace Chick; Research Associate). Neuroscience Program members interested in generating transgenic, knock-out and
knock-in mice should contact Mark Dell'Acqua. Due to the highly specialized nature of the work only a small number of gene-targeted/transgenic mouse projects will be under taken at any given time. Selection of these projects will be based on proposals
from investigators that will be evaluated by the Core B advisory panel (Wallace Chick, Brian Parr, Trevor Williams, Mark Dell'Acqua, and Diego Restrepo). Please contact
Core B director Mark Dell'Acqua for more details.
- Generation of Viral Vectors for Infection of Neurons/Glia (Jerry Schaack, Coordinator). This aspect of the core is open for business. Individuals should contact Jerry Schaak.
Angie Ribera, DirectorThe NINDS P30 Center Zebrafish Core has three Specific Aims:
- Create transgenic lines of zebrafish that express different fluorescent proteins in specific populations of pre- and postsynaptic neurons
- Create transgenic zebrafish strains that express genetically-encoded calcium indicator dyes in specific neuronal populations
- Maintain transgenic and wild type zebrafish strains for the UCD neuroscience community
During the first year of the grant, the fish facility at the Fitzsimons campus is being optimized and we are focusing on Aim 3. In subsequent years, Aims 1 and 2 will be a priority. The Core can cover the expenses associated with breeding wild type fish
for pilot experiments. If you would like to use zebrafish embryos for pilot experiments, please contact Angie Ribera for further information.
lines express GFP in specific neuronal subpopulations. In the Figure, GFP expression is driven by the flh promoter. (This transgenic line was developed by Drs. Marnie Halpern and Josh Gamse - Carnegie Institute of Washington). At 48 hours post fertilization,
GFP expression (green) is present in specific populations of motor neurons that extend axons either ventrally (down, asterisks) or dorsally (up, arrowhead). A subset of the axons also expresses the zn-8 epitope (red). Zn-8 is a marker for axons
of later-born (secondary) motor neurons.
Scale Bar: 25 µm.
John Caldwell, Director
The Neuroscience core machine shop is located in room NG003 on the ground floor of Building 500 (northwest corner) at the Anschutz Medical Campus (View Map). The shop's equipment and staff are there to meet the custom
machining needs of the faculty, staff and students of the Neuroscience program.
The shop machinery includes:
- 2 milling machines with 9" x 42" tables (1 with digital readout)
- 1 CNC milling machine (full 3-axis automation)
- 2 Lathes
- 2 wood/metal-cutting band saws
- 1 table saw
- 1 chop saw
- 2 grinding machines
- 1 drill press
- 1 12" disk/6" belt sander
- 1 52" shear
- Extensive tooling
& precision measuring devices
The shop stocks a modest amount of material (acrylic, aluminum, brass, hardware) for small projects, and this material is available to all. However, the cost of material
not routinely kept on-hand is the responsibility of the laboratory requesting the project. Shop labor is provided at no cost to members of the Neuroscience Program.
The shop is staffed by one machinist 24 hours/week although there are currently
no routine hours of operation. Contact Michael Hall at 303-724-1335 or, preferably, by e-mail at Michael.Hall@ucdenver.edu to discuss a project, to arrange a meeting or with questions.
Other Facilities and Cores
Charles C. Gates Center for Regenerative Medicine and Stem Cell Biology
The Gates Center for Regenerative Medicine is a world-class facility located on the Anschutz Medical Campus, the largest new biomedical and clinical campus in the United States. Its first-rate laboratory, as well as its ability to conduct on-site clinical trials has drawn some of the nation’s top talent in stem cell research.
Learn more about the Gates Center.
Colorado Clinical and Translational Sciences Institute
The Colorado Clinical & Translational Sciences Institute (CCTSI) is the academic home to help transform the clinical and translational research and training efforts at the University of Colorado Anschutz Medical Campus and affiliated institutions. The CCTSI was created in 2008 with funding from the Clinical and Translational Science Award (CTSA) initiative of the National Institutes of Health (NIH).
Learn more about the CCTSI.
Research Core Facilities
The University of Colorado School of Medicine is fully equipped with specialized instrumentation and core facilities required to conduct contemporary biomedical investigations. Core facilities include:
- Advanced Light Microscopy Core
- Biological Mass Spectrometry Facility
- Biostatistics and Bioinformatics Shared Resource (BBSR)
- Colorado Translational Research Imaging Center (C-TRIC)
- Cytogenetics Service
- Flow Cytometry Shared Resource Facility
- Genomics and Microarray Core
- Human Immune Monitoring Shared Resource (HIMSR)
- NMR Structural Biology Shared Resource Facility
- Peptide and Protein Chemistry Core Facility
- Protein Production/MoAB/Tissue Culture Core
- Structural Biology Core
- Transgenic and Gene Targeting Core
- Xray Crystallography Structural Biology Shared Resource
Learn more about research centers and institutes.
Other Centers and Institutes
CU Anschutz provides state of the art research, information and clinical care with experts working in dozens of centers and institutes focused on diverse areas of study, such as Alzheimer’s Disease, childhood diabetes, mental health, women’s health, aging, stem cell biology, multiple sclerosis and cancer.
All CU Anschutz Centers, Institutes, and Affiliates.