Automated Solid-Phase Peptide Synthesizers
• AAPPTec Focus XC-4RV for Boc- and Fmoc-chemistry
• CEM Liberty Microwave for Fmoc-chemistry
Analytical and Preparative HPLC Systems
• Agilent 1100 and 1200 HPLC analytical systems
• Agilent 1100 LC/Electrospray MS system
• Beckman System Gold preparative HPLC system (10 ml/min max.)
• Sharp Semi-prep Gradient Purification System (5 to 50 ml/min)
HF Cleavage apparatus for Boc-chemistry
Peptide Synthesis (Solid-phase / solution phase)
Peptides can be synthesized using L- and/or D-amino acids or unusual amino acids. Boc-, Fmoc- or a combination of these synthesis chemistries can be used.
Peptide Purification (Reversed-phase HPLC)
Our facility only provides synthetic peptides at purities greater than 95% at the milligram to gram scale. All peptides are analyzed for purity by analytical reversed-phase HPLC and characterized by LC-MS (electrospray mass spectrometry).
Peptide Characterization (LC/MS)
See the rates and policies tab for possible modifications. If you don't see the modificiation you want, feel free to inquire.
Peptide Conjugation (polyclonal/monoclonal antibody production)
We can couple synthetic peptides to carrier proteins for production of polyclonal or monoclonal antibodies. We generally couple to Keyhole limpet hemocyanin (KLH) for antibody production and Bovine serum albumin (BSA) for screening antibodies by ELISA.
Quantitation (amino acid analysis)
Our staff has over 100 years of experience in peptide design and synthesis, and we are happy to discuss your order with you at no charge. Customers will receive a quotation prior to beginning production to assess costs. The costs of synthesis are dependent on peptide length and the incorporated modifications. Some bulk discounts may be available depending on the project.
Minimum charge for synthesis ($250)
0.1 mM scale: $25 per residue
0.25 mM scale: $40 per residue
All peptides are cleaved from the resin, resolubilized in appropriate solvent, and analyzed by reversed phase HPLC and LC-MS
Preparative HPLC purification of synthetic peptides: $250 per peptide (depending on scale of synthesis)
Additional modification of peptides:
Incorporation of phosphorylated Ser or Thr
Incorporation of D-amino acids or other unusual amino acids
Biotin (C-terminal or N-terminal)
Conjugation to KLH or BSA
AMINO ACID ANALYSIS
$40 per sample
LC-Mass determination of proteins, peptides, etc: $15 per sample
Contact InformationPeptide and Protein Chemistry Facility University of Colorado Anschutz Medical Campus Bldg. RC-1 South, Room L18-1308 12801 E. 17th Avenue, Mail Stop 8101Aurora, CO 80045
Dr. Robert S. Hodges, DirectorPhone: 303-903-5591Fax: 303-724-3249Office: UCDenver - Anschutz Medical Campus, Bldg. RC-1S, Rm. L9121Robert.Hodges@ucdenver.edu
Dr. Lajos Gera, Research Associate ProfessorPhone: 303-724-3336Office: UCDenver - Anschutz Medical Campus, Bldg. RC-1S, Rm. L1308Lajos.Gera@ucdenver.edu
Timothy Davis, Professional
Office: UCDenver - Anschutz Medical Campus, Bldg. RC-1S, Rm. L1308