|Group||Arctica*||L120C**||Manager Fee (Per hour)||Data analysis (Per hour)***|
|$ 25.00||$ 20.00||$ 50.00||$100.00|
|$ 15.00||$ 10.00|| || |
|$ 70.00||$ 40.00||$ 60.00||$ 100.00|
|$ 35.00||$ 20.00|| || |
|$ 100.00||$ 60.00||$ 80.00||$ 100.00|
|$ 50.00||$ 30.00|| || |
|$ 300.00||$ 250.00||$ 100.00||$ 300.00|
|$ 200.00||$ 150.00|| || |
*Users are billed at Rate 1
for the first 24 hours of Arctica use in a month. Users are billed at Rate 2
after 24 hours.
**Users are billed at Rate 1
for the first 6 hours of L120C use in a month. Users are billed at Rate 2
after 6 hours.
***Data analysis is only offered where time is available
Any projects where Anschutz CryoEM staff prepare samples, grids, or do analysis of resulting images are considered to be collaborations. For collaboration work with the CryoEM facility that results in publication, CryoEM staff members should be listed as co-authors.
For data collection-only services, on user-provided ready-to-image cryo grids, if CryoEM staff enabled customization of approaches or made significant intellectual input or overcame extra difficulties (such as multiple loads of grids to find a usable one instead of a single load of up to 8 grids for one sample) to make the work possible, they should be listed as co-authors. Otherwise, the following acknowledgement should be included in the publication:
1. When describing data collection in the Materials and Methods or equivalent section, please mention that data was collected on a 200 kV FEI Talos Arctica microscope or a 120 kV FEI Talos L120C located at the Anschutz Medical Campus.
2. In the Acknowledgement section of the paper, please acknowledge help from the Anschutz CryoEM facility staff.
After the paper is published, please send a link to the paper as well as one representative figure to the director of the Anschutz CryoEM facility.Billing rates subject to change
EMAN2: A broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. EMAN2 is capable of processing very large data sets (>100,000 particle) very efficiently (up to 20x faster than EMAN1).
RELION: RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheresat the MRC Laboratory of Molecular Biology. Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB. A more detailed description of its implementation is given in Scheres (2012) JSB. If RELION is useful in your work, please cite at least one of these papers.